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Glycerol-based · Ready to order

SP6 RNA Polymerase
Glycerol-based.

A highly processive enzyme that shows specificity for its double-stranded promoter sequence (5′ ATTTAGGTGACACTATAG 3′) and catalyzes the 5′→3′ synthesis of RNA. Purified using our ArtPure™ proprietary purification process, yielding a >99% pure, animal origin-free product free of contaminating proteinases, RNases and DNases — with no added enzyme inhibitors.

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>99%Enzyme purity
AOFAnimal-origin free
−20°CStable
CoACertificate with every batch
01 IVT Protocol Suggested IVT protocol for optimized ArtPure™ SP6 activity. 02 Enzyme Quality ArtPure™ SP6 is free of contaminants, ensuring high yield of pure RNA. 03 Product Information Optimal storage conditions ensure prolonged enzyme function. 04 Order Get in touch to get more information and a quote.
IVT Protocol

Suggested IVT protocol.

Assemble the following reaction at room temperature. The volumes can be scaled according to the application.

ComponentAmount
Linearized DNA template500 ng
NTP1 mM each
RNase inhibitor1 unit
SP6 RNA Polymerase1 µl
Nuclease-free waterto 10 µl
1Incubate the reaction 1 hour at 37°C.
2To remove the DNA template, add 1 U of DNase I and incubate at 37°C for 15 minutes.
3Inactivate the DNase I by adding 5 mM of EDTA, incubate at 70°C for 10 minutes.
Enzyme Quality

Superior yield, integrity and purity.

ArtPure™ SP6 RNA Polymerase outperforms industry standards across yield, RNA integrity and dsRNA by-products — verified by head-to-head comparison.

Purity

SP6 RNA Polymerase purity by SDS-PAGE

Superior RNA Yield

SP6 RNA Polymerase yield comparison vs competitors

Low dsRNA By-products

SP6 RNA Polymerase dsRNA by-products comparison vs competitors

Definitions

Purity: Evaluated by SDS-PAGE, where the integrated peak area of the RNA Polymerase is compared to other protein peaks in the same lane.
RNA yield: Quantification of total RNA products after in vitro transcription and RNA purification, compared to leading suppliers.
dsRNA by-products: Quantification of double-stranded RNA by-products using a dsRNA-specific ELISA, compared to leading suppliers.
📄 Certificate of Analysis — COA_Z061_092024_v1
Product Information

Storage & handling.

Optimal storage conditions ensure prolonged enzyme function.

Storage upon receipt

Our SP6 RNA Polymerase is stable at −20°C for up to 12 months in the glycerol-based supplied buffer. For extended storage, we recommend aliquoting in smaller volumes, freezing at −80°C and avoiding repeated freeze-thaw cycles.

Storage buffer

50 mM Tris-HCl, 100 mM NaCl, 20 mM DTT, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9.

Order

Pack sizes & pricing.

Get in touch to get more information and a quote.

ProductSKUPack size
SP6 RNA Polymerase (Glycerol-based) Z0611 3,000 U Contact us →
SP6 RNA Polymerase (Glycerol-based) Z0613 10,000 U Contact us →
SP6 RNA Polymerase (Glycerol-based) Z061X Custom Contact us →

Need the T7 promoter instead?

Our T7 RNA Polymerase is available in glycerol-based and lyophilized, room-temperature-shippable formats.

See T7 RNA Polymerase Request a quote