Complete, ready-to-use kits for reproducible in vitro transcription — optimised buffers, nucleotides, control template and ArtPure™ enzymes in a single workflow, including capped mRNA synthesis for downstream translation studies.
ARCA kits available in 25 or 50 reactions; Co Cap™ A kits in 10 or 25 reactions. All kits are stable at −20°C for up to 12 months. Get in touch for pricing or bulk quantities.
Flexible, high-yield RNA synthesis via in vitro transcription with ArtPure™ T7 RNA Polymerase. Each reaction yields roughly 100–120 µg of RNA after a 2-hour incubation — suitable for RNA structure and function studies, microinjection, transfection, in situ hybridization, and in vitro translation.
High-yield capped mRNA via in vitro transcription. ARCA variants (Cap-0, 20–25 µg/rxn) available with T7 or SP6 polymerase; Co-Cap™ A variants (Cap-1, 160–180 µg/rxn) with T7 polymerase — all with or without m¹Ψ nucleotides. Validated in zebrafish and salmon.
ARCA-capped (Cap-0) mRNA, 20–25 µg per reaction. T7 and SP6 polymerase options; nucleotides and ARCA available as a 5× mix or individual vials for modified-nucleotide flexibility. Validated in zebrafish and salmon.
ARCA cap combined with N¹-methylpseudouridine (m¹Ψ). Improves transcript stability, minimizes immunogenicity, and enhances translation efficiency compared to unmodified ARCA mRNA.
Exceptional yields of Cap-1 mRNA — 160–180 µg per reaction via co-transcriptional capping. Enhanced translational efficiency and reduced immune stimulation vs ARCA-capped mRNA. Superior transfection performance.
Co-Cap™ A with N¹-methylpseudouridine (m¹Ψ) for maximum performance: exceptional yield, promoted transcript stability, enhanced translation efficiency and reduced immunogenicity — all in a single co-transcriptional reaction.
High-yield capped mRNA production validated in vivo in zebrafish embryos. ARCA Cap-0 capping in every batch, available with T7 or SP6 RNA Polymerase — 30–35 µg capped mRNA per reaction.
Produces 30–35 µg of capped mRNA per reaction via T7 RNA Polymerase with ARCA (Cap-0) capping. Nucleotides and cap analog available as a 5X mix or individual vials for modified-nucleotide incorporation.
SP6 RNA Polymerase variant, producing 20–25 µg of capped mRNA per reaction with ARCA capping for reliable translation efficiency — validated in vivo via eGFP expression in zebrafish.
Each kit pairs an ArtPure™ polymerase with carefully matched buffers and nucleotides, so you spend less time troubleshooting and more time getting usable RNA.
Enzyme mix, NTPs, buffer and control template arrive matched and pre-optimised.
>99% pure, animal origin-free polymerase, free of proteinase, RNase and DNase contamination.
Consistent ARCA capping in every batch, for dependable translation efficiency from kit to kit.
Tell us what you're transcribing and how it'll be used downstream — we'll recommend the right kit and pack size.