Ready to order

IVT & transcription
kits.

Complete, ready-to-use kits for reproducible in vitro transcription — optimised buffers, nucleotides, control template and ArtPure™ enzymes in a single workflow, including capped mRNA synthesis for downstream translation studies.

>99%Enzyme purity
5-in-1Complete kit
3Pack sizes per kit
CoACertificate with every batch

Available kits.

ARCA kits available in 25 or 50 reactions; Co Cap™ A kits in 10 or 25 reactions. All kits are stable at −20°C for up to 12 months. Get in touch for pricing or bulk quantities.

T7 RNA Synthesis Kit

T7 RNA Synthesis Kit

Unmodified RNA

Flexible, high-yield RNA synthesis via in vitro transcription with ArtPure™ T7 RNA Polymerase. Each reaction yields roughly 100–120 µg of RNA after a 2-hour incubation — suitable for RNA structure and function studies, microinjection, transfection, in situ hybridization, and in vitro translation.

T7 RNAP enzyme mix Individual NTPs Reaction buffer Control DNA template DNase I + EDTA
25 reactionsKS07025
50 reactionsKS07050
NorthScribe Transcription Kit

NorthScribe™ Transcription Kit

Capped mRNA

High-yield capped mRNA via in vitro transcription. ARCA variants (Cap-0, 20–25 µg/rxn) available with T7 or SP6 polymerase; Co-Cap™ A variants (Cap-1, 160–180 µg/rxn) with T7 polymerase — all with or without m¹Ψ nucleotides. Validated in zebrafish and salmon.

T7 or SP6 RNAP Cap analog included 5× NTP mix or individual vials Reaction buffer Control template
4 variants
ARCA · Cap-0

NorthScribe™ ARCA

ARCA-capped (Cap-0) mRNA, 20–25 µg per reaction. T7 and SP6 polymerase options; nucleotides and ARCA available as a 5× mix or individual vials for modified-nucleotide flexibility. Validated in zebrafish and salmon.

25 rxn (T7)AKT07025
50 rxn (T7)AKT07050
25 rxn (SP6)AKT06025
50 rxn (SP6)AKT06050
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m¹Ψ-ARCA · Cap-0

NorthScribe™ m¹Ψ-ARCA

ARCA cap combined with N¹-methylpseudouridine (m¹Ψ). Improves transcript stability, minimizes immunogenicity, and enhances translation efficiency compared to unmodified ARCA mRNA.

25 rxn (T7)AKT17025
50 rxn (T7)AKT17050
25 rxn (SP6)AKT16025
50 rxn (SP6)AKT16050
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Co-Cap™ A · Cap-1

NorthScribe™ Co-Cap™ A

Exceptional yields of Cap-1 mRNA — 160–180 µg per reaction via co-transcriptional capping. Enhanced translational efficiency and reduced immune stimulation vs ARCA-capped mRNA. Superior transfection performance.

10 rxn (T7)CKT07010
25 rxn (T7)CKT07025
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m¹Ψ-Co-Cap™ A · Cap-1

NorthScribe™ m¹Ψ-Co-Cap™ A

Co-Cap™ A with N¹-methylpseudouridine (m¹Ψ) for maximum performance: exceptional yield, promoted transcript stability, enhanced translation efficiency and reduced immunogenicity — all in a single co-transcriptional reaction.

10 rxn (T7)CKT17010
25 rxn (T7)CKT17025
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ZebraScribe Transcription Kit

ZebraScribe™ Transcription Kit

Capped mRNA

High-yield capped mRNA production validated in vivo in zebrafish embryos. ARCA Cap-0 capping in every batch, available with T7 or SP6 RNA Polymerase — 30–35 µg capped mRNA per reaction.

T7 or SP6 RNAP ARCA cap analog 5X NTP mix Reaction buffer Control template
2 variants
T7 Polymerase

ZebraScribe™ T7

Produces 30–35 µg of capped mRNA per reaction via T7 RNA Polymerase with ARCA (Cap-0) capping. Nucleotides and cap analog available as a 5X mix or individual vials for modified-nucleotide incorporation.

25 reactionsSKU ZKT07025
50 reactionsSKU ZKT07050
View details → Request a quote →
SP6 Polymerase

ZebraScribe™ SP6

SP6 RNA Polymerase variant, producing 20–25 µg of capped mRNA per reaction with ARCA capping for reliable translation efficiency — validated in vivo via eGFP expression in zebrafish.

25 reactionsSKU ZKT06025
50 reactionsSKU ZKT06050
View details → Request a quote →

Everything you need, optimised to work together.

Each kit pairs an ArtPure™ polymerase with carefully matched buffers and nucleotides, so you spend less time troubleshooting and more time getting usable RNA.

One workflow, fewer reagents

Enzyme mix, NTPs, buffer and control template arrive matched and pre-optimised.

ArtPure™ enzyme inside

>99% pure, animal origin-free polymerase, free of proteinase, RNase and DNase contamination.

Reliable cap orientation (ZebraScribe)

Consistent ARCA capping in every batch, for dependable translation efficiency from kit to kit.

Not sure which kit fits your workflow?

Tell us what you're transcribing and how it'll be used downstream — we'll recommend the right kit and pack size.