Unmodified RNA · Ready to order

T7 RNA Synthesis
Kit.

Designed to provide flexible, high-yield synthesis of RNA via in vitro transcription with ArtPure™ T7 RNA Polymerase. The synthesized RNA is suited for RNA structure and function studies, microinjection, transfection, in situ hybridization and in vitro translation. Available in 25, 50 or 200 reactions, with each reaction yielding roughly 100–120 µg of RNA after a 2-hour incubation.

Request a quote ← Back to Kits

IVT Protocol

Suggested IVT protocol.

Assemble the following reaction at room temperature. The volumes can be scaled according to the application.

Component	Recommended amount or volume
Linearized DNA template*	0.1–1 µg
10X reaction buffer	2 µl
ATP	1.5 µl
CTP	1.5 µl
GTP	1.5 µl
UTP	1.5 µl
Enzyme mix	2.5 µl
Nuclease-free water	to 20 µl

*0.1 µg of PCR product or 1 µg of restriction digest template.

1Incubate the reaction 2 hours at 37°C.

2To remove the DNA template, add 1 µl of DNase I and incubate at 37°C for 15 minutes.

3Inactivate the DNase I by adding 2 µl EDTA (50 mM), incubate at 70°C for 10 minutes.

Enzyme Quality

Built for clean, reliable in vitro transcription.

ArtPure™ T7 is free of contaminants, ensuring high yield of pure RNA. Every batch is evaluated across purity, activity and contaminant panels.

Purity

RNases

Activity

Proteinases

DNases

Definitions

Activity (U/µl): One enzyme unit incorporates 1 nmol of soluble RNA nucleotides into insoluble polymeric RNA per hour at 37°C.

Purity: Evaluated by SDS-PAGE, where the integrated peak area of the RNA Polymerase is compared to other protein peaks in the same lane.

Transcriptional activity: Quantification of total RNA products after in vitro transcription and RNA purification.

Full-length transcripts (%): Amount of full-length transcripts produced relative to amount of total RNA, calculated using the area under the curve (AUC) from electropherogram obtained with a 2100 Bioanalyzer RNA 6000 Nano kit.

Proteinases: Assessed using Pierce™ Fluorescent Protease Assay Kit (ThermoFisher Scientific) following the manufacturer's guidelines.

RNases: Assessed using the RNase Alert kit (Integrated DNA Technologies), following the manufacturer's guidelines.

Double-stranded endonucleases: 2.5 µl of the T7 RNAP are incubated with 1 µg of supercoiled plasmid DNA for 0 hours, 4 hours at 37°C and 16 hours at room temperature.

Double-stranded exonucleases: 2.5 µl of the T7 RNAP are incubated with 0.5 µg of linearized double-stranded DNA substrate for 0 hours, 4 hours at 37°C and 16 hours at room temperature.

Single-stranded exonucleases: 2.5 µl of the T7 RNAP are incubated with 1 µg of ssDNA substrate (calf-thymus DNA) for 0 hours, 4 hours at 37°C and 16 hours at room temperature.

📄 Certificate of Analysis — COA_Z071_092024_v1

Product Information

Storage & handling.

Optimal storage conditions ensure prolonged enzyme function.

Storage upon receipt

The components of the kit are stable at −20°C for up to 12 months. For extended storage, we recommend aliquoting in smaller volumes, freezing at −80°C and avoiding repeated freeze-thaw cycles.

Storage buffer

50 mM Tris-HCl, 100 mM NaCl, 20 mM DTT, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9.

Product	SKU	Pack size
T7 RNA Synthesis Kit	KS07025	25 reactions	Contact us →
T7 RNA Synthesis Kit	KS07050	50 reactions	Contact us →
T7 RNA Synthesis Kit	KS070200	200 reactions	Contact us →

T7 RNA Synthesis
Kit.

Suggested IVT protocol.