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Glycerol-based · Ready to order

T7 RNA Polymerase
Glycerol-based.

A highly processive enzyme that shows specificity for its double-stranded promoter sequence (5′ TAATACGACTCACTATAG 3′) and catalyzes the 5′→3′ synthesis of RNA. Purified using our ArtPure™ proprietary purification process, yielding a >99% pure, animal origin-free product free of contaminating proteinases, RNases and DNases — with no added enzyme inhibitors.

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>99%Enzyme purity
AOFAnimal-origin free
−20°CStable
CoACertificate with every batch
01 IVT Protocol Suggested IVT protocol for optimized ArtPure™ T7 activity. 02 Enzyme Quality ArtPure™ T7 is free of contaminants, ensuring high yield of pure RNA. 03 Product Information Optimal storage conditions ensure prolonged enzyme function. 04 Order Get in touch to get more information and a quote.
IVT Protocol

Suggested IVT protocol.

Assemble the following reaction at room temperature. The volumes can be scaled according to the application.

ComponentAmount
Linearized DNA template500 ng
NTP1 mM each
RNase inhibitor1 unit
T7 RNA Polymerase1 µl
Nuclease-free waterto 10 µl
1Incubate the reaction 1 hour at 37°C.
2To remove the DNA template, add 1 U of DNase I and incubate at 37°C for 15 minutes.
3Inactivate the DNase I by adding 5 mM of EDTA, incubate at 70°C for 10 minutes.
Enzyme Quality

Superior yield, integrity and purity.

ArtPure™ T7 RNA Polymerase outperforms industry standards across yield, RNA integrity and dsRNA by-products — verified by head-to-head comparison.

Purity

T7 RNA Polymerase purity by SDS-PAGE

Superior RNA Yield

T7 RNA Polymerase yield comparison vs competitors

Low dsRNA By-products

T7 RNA Polymerase dsRNA by-products comparison vs competitors

Definitions

Activity (U/µl): One enzyme unit incorporates 1 nmol of soluble RNA nucleotides into insoluble polymeric RNA per hour at 37°C.
Purity: Evaluated by SDS-PAGE, where the integrated peak area of the RNA Polymerase is compared to other protein peaks in the same lane.
RNA yield: Quantification of total RNA products after in vitro transcription and RNA purification, compared to leading suppliers.
dsRNA by-products: Quantification of double-stranded RNA by-products using a dsRNA-specific ELISA, compared to leading suppliers.
📄 Certificate of Analysis — COA_Z071_092024_v1
Product Information

Storage & handling.

Optimal storage conditions ensure prolonged enzyme function.

Storage upon receipt

Our T7 RNA Polymerase is stable at −20°C for up to 12 months in the glycerol-based supplied buffer. For extended storage, we recommend aliquoting in smaller volumes, freezing at −80°C and avoiding repeated freeze-thaw cycles.

Storage buffer

50 mM Tris-HCl, 100 mM NaCl, 20 mM DTT, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9.

Order

Pack sizes & pricing.

Get in touch to get more information and a quote.

ProductSKUPack size
T7 RNA Polymerase (Glycerol-based) Z0711 5,000 U Contact us →
T7 RNA Polymerase (Glycerol-based) Z0713 15,000 U Contact us →
T7 RNA Polymerase (Glycerol-based) Z071X Custom Contact us →

Need a room-temperature format?

Our lyophilized T7 RNA Polymerase ships without cold chain — same ArtPure™ purity, with simpler logistics.

See lyophilized format ← Back to RNA Polymerases